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Culture conditions and salt effects on essential oil composition of sweet marjoram (Origanum majorana) from Tunisia.

Acta pharmaceutica (Zagreb, Croatia) (2012-07-04)
Olfa Baâtour, Imen Tarchoune, Hela Mahmoudi, Nawel Nassri, Wissal Abidi, Rym Kaddour, Ghaith Hamdaoui, Mouhiba Ben Nasri-Ayachi, Mohtar Lachaâl, Brahim Marzouk
ABSTRACT

O. majorana shoots were investigated for their essential oil (EO) composition. Two experiments were carried out; the first on hydroponic medium in a culture chamber and the second on inert sand in a greenhouse for 20 days. Plants were cultivated for 17 days in hydroponic medium supplemented with NaCl 100 mmol L⁻¹. The results showed that the O. majorana hydroponic medium offered higher essential oil yield than that from the greenhouse. The latter increased significantly in yield (by 50 %) under saline constraint while it did not change in the culture chamber. Under greenhouse conditions and in the absence of salt treatment, the major constituents were terpinen-4-ol and trans-sabinene hydrate. However, in the culture chamber, the major volatile components were cis-sabinene hydrate and terpinen-4-ol. In the presence of NaCl, new compounds appeared, such as eicosane, spathulenol, eugenol, and phenol. In addition, in the greenhouse, with or without salt, a very important change of trans-sabinene hydrate concentration in EO occurred, whereas in the culture chamber change appeared in cis-sabinene hydrate content.

MATERIALS
Product Number
Brand
Product Description

Terpinen 4-ol, primary reference standard
Sigma-Aldrich
4-Carvomenthenol, ≥95%, FCC, FG
Sigma-Aldrich
4-Carvomenthenol, natural, ≥95%, FG