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  • Precision-cut liver slices to investigate responsiveness of deep-sea fish to contaminants at high pressure.

Precision-cut liver slices to investigate responsiveness of deep-sea fish to contaminants at high pressure.

Environmental science & technology (2012-08-21)
Benjamin Lemaire, Cathy Debier, Pedro Buc Calderon, Jean Pierre Thomé, John Stegeman, Jarle Mork, Jean François Rees
ABSTRACT

While deep-sea fish accumulate high levels of persistent organic pollutants (POPs), the toxicity associated with this contamination remains unknown. Indeed, the recurrent collection of moribund individuals precludes experimental studies to investigate POP effects in this fauna. We show that precision-cut liver slices (PCLS), an in vitro tool commonly used in human and rodent toxicology, can overcome such limitation. This technology was applied to individuals of the deep-sea grenadier Coryphaenoides rupestris directly upon retrieval from 530-m depth in Trondheimsfjord (Norway). PCLS remained viable and functional for 15 h when maintained in an appropriate culture media at 4 °C. This allowed experimental exposure of liver slices to the model POP 3-methylcholanthrene (3-MC; 25 μM) at levels of hydrostatic pressure mimicking shallow (0.1 megapascal or MPa) and deep-sea (5-15 MPa; representative of 500-1500 m depth) environments. As in shallow water fish, 3-MC induced the transcription of the detoxification enzyme cytochrome P4501A (CYP1A; a biomarker of exposure to POPs). This induction was diminished at elevated pressure, suggesting a limited responsiveness of C. rupestris toward POPs in its native environment. This very first in vitro toxicological investigation on a deep-sea fish opens the route for understanding pollutants effects in this highly exposed fauna.

MATERIALS
Product Number
Brand
Product Description

Supelco
3-Methylcholanthrene solution, 100 μg/mL in acetonitrile, PESTANAL®, analytical standard
Sigma-Aldrich
3-Methylcholanthrene, 98%
Supelco
3-Methylcholanthrene, analytical standard