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Conversion of 11-ketoandrostenedione to 11-ketotestosterone by blood cells of six fish species.

General and comparative endocrinology (1990-01-01)
I Mayer, B Borg, R Schulz
ABSTRACT

Blood cells from Baltic salmon, Salmo salar, three-spined stickleback, Gasterosteus aculeatus, eel pout, Zoarces viviparus, crucian carp, Carassius carassius, African catfish, Clarias gariepinus, and reedfish, Calamoichthys calabaricus, were incubated with tritiated 11 beta-hydroxyandrostenedione (OHA) or 11-ketoandrostenedione (OA). In all fish there was conversion of OA to 11-ketotestosterone (OT), indicating that 17 beta-hydroxysteroid dehydrogenase activity was present in the blood cells. On the other hand, OHA was not converted to 11 beta-hydroxytestosterone in any fish. The addition of serum to the incubates largely prevented the OA-OT conversion by salmon blood cells.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Adrenosterone, 98%