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Effect of dietary copper on the growth performance, non-specific immunity and resistance to Aeromonas hydrophila of juvenile Chinese mitten crab, Eriocheir sinensis.

Fish & shellfish immunology (2013-02-21)
Shengming Sun, Jianguang Qin, Na Yu, Xianping Ge, Haibo Jiang, Liqiao Chen
ABSTRACT

An 8-week feeding trial was conducted to determine the dietary copper (Cu) on growth performance and immune responses of juvenile Chinese mitten crab Eriocheir sinensis. Six semi-purified diets with six copper levels (1.88, 11.85, 20.78, 40.34, 79.56 and 381.2 mg kg(-1) diet) of CuSO4·5H2O were fed to E. sinensis (0.45 ± 0.01 g). Each diet was fed to the crab in five replicates. The crab fed diets with 20.78 and 40.34 mg Cu kg(-1) diet had significantly greater weight gain and hemolymph oxyhemocyanin content than those fed diets with 1.88 and 381.2 mg Cu kg(-1) diet. Survival rates of crab were not significantly different between all treatment groups. The activities of copper-zinc superoxide dismutase (Cu-Zn SOD), phenoloxidase (PO), and total hemocyte count (THC) significantly increased when the supplementation of dietary copper reached 20.78-40.34 mg Cu kg(-1) diets. In the bacteria challenge experiment with Aeromonas hydrophila, survival rates significantly increased and reached a plateau when the dietary copper increased from 1.88 to 40.34 mg kg(-1), whereas significantly decreased when the dietary copper increased from 40.34 to 381.2 mg kg(-1). This study indicates that the level of dietary copper is important in regulating growth and immune response in crab.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Hemocyanin from Limulus polyphemus hemolymph, Type VIII, lyophilized powder, Protein ≥85 % by biuret