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A FLIPR assay for evaluating agonists and antagonists of GPCR heterodimers.

Methods in molecular biology (Clifton, N.J.) (2013-03-16)
Jessica H Harvey, Richard M van Rijn, Jennifer L Whistler
ABSTRACT

Calcium signaling plays a major role in the function of cells. Measurement of intracellular calcium mobilization is a robust assay that can be performed in a high-throughput manner to study the effect of compounds on potential drug targets. Pharmaceutical companies frequently use calcium signaling assays to screen compound libraries on G-protein-coupled receptors (GPCRs). In this chapter we describe the application of FLIPR technology to the evaluation of GPCR-induced calcium mobilization. We also include the implications of GPCR hetero-oligomerization and the identification of heteromeric receptors as novel drug targets on high-throughput calcium screening.

MATERIALS
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Product Description

Supelco
Naltrexone solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

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