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Stabilization of a primary loop in myosin subfragment 1 with a fluorescent crosslinker.

Proceedings of the National Academy of Sciences of the United States of America (1985-03-01)
D Mornet, K Ue, M F Morales
ABSTRACT

A bifunctional fluorescent alkylating agent, dibromobimane, has been used to stabilize a preexisting primary loop in myosin subfragment 1 (S-1). The crosslink achieved joins Cys-707 (called sulfhydryl group "SH1") of the 20-kDa domain (formerly called "20K" domain) with a thiol of the 50-kDa domain and seems to place the dibromobimane near the ATP-perturbable tryptophan.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Dibromobimane, BioReagent, suitable for fluorescence, ≥95.0% (CHN)

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