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Short-chain phospholipids enhance amphipathic peptide-mediated gene transfer.

Biochemical and biophysical research communications (1995-12-05)
J Y Legendre, A Supersaxo
ABSTRACT

Addition of short-chain phospholipids to the gramicidin S-DNA-dioleoyl phosphatidylethanolamine complex enhanced up to 6-fold beta-galactosidase expression in several cell-lines in vitro. Among the compounds tested, the most potent in enhancing transfection were the dicapryl- and the dicapryloyl phosphatidylcholine. In contrast, no significant enhancement of transfection was seen when short-chain phospholipids were mixed with cationic lipids. Short-chain phospholipid and gramicidin S may act simultaneously on the cell membrane to enhance gene transfer, yet resulting in no toxicity.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
1,2-Didecanoyl-sn-glycero-3-phosphocholine, ≥99% (TLC)