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Identification of a family of closely related human ubiquitin conjugating enzymes.

The Journal of biological chemistry (1995-12-22)
J P Jensen, P W Bates, M Yang, R D Vierstra, A M Weissman
ABSTRACT

Two very closely related human E2 ubiquitin conjugating enzymes, UbfH5B and UbcH5C, have been identified. These enzymes are products of distinct genes and are 88-89% identical in amino acid sequence to the recently described human E2, UbcH5 (now designated UbcH5A), UbcH5A-C are homologous to a family of five ubiquitin conjugating enzymes from Arabidopsis thaliana, AtUBC8-12. They are also closely related to Saccharomyces cerevisiae ScUBC4 and ScUBC5, which are involved in the stress response, and play a central role in the targeting of short-lived regulatory proteins for degradation. mRNAs encoding UbcH5A-C were co-expressed in all cell lines and tissues evaluated, with UbcH5C transcripts generally expressed at the highest levels. Analysis of Southern blots suggests that there are likely to be other related members of this family. Both UbcH5B and UbcH5C form thiol ester adducts with ubiquitin, and have activities similar to UbcH5A and AtUBC8 in the conjugation of ubiquitin to target proteins in the presence of the human ubiquitin protein ligase E6-AP. These results establish the existence of a highly conserved, and widely expressed, family of human ubiquitin conjugating enzymes.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
UbcH5b Conjugating Enzyme, 100 µg, The His6-tagged fusion protein of UbcH5b is charge & support ubiquitinylation in vitro. The His6-tagged UbcH5 family members all appear to form thiol ester conjugates with ubiquitin at a similar rate under similar conditions.
Sigma-Aldrich
UbcH5c Conjugating Enzyme, 100 µg, The His6-tagged fusion protein of UbcH5c is charge & support ubiquitinylation in vitro. The His6-tagged UbcH5 family members all appear to form thiol ester conjugates with ubiquitin at a similar rate under similar conditions.
Sigma-Aldrich
Ubiquitin-Carrier Protein H5b human, ≥95% (SDS-PAGE), solution

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