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Sensitive high-performance liquid chromatographic method for determination of captopril in plasma.

Pharmaceutica acta Helvetiae (1999-08-12)
M Amini, A Zarghi, H Vatanpour
ABSTRACT

A rapid, simple and sensitive high-performance liquid chromatographic method for the determination of captopril in plasma has been developed. Captopril is derivatized with a new reagent, 2-bromo-2'-acetonaphthone to form a product that showed ultraviolet-absorbing properties. For plasma samples, the protein was removed with 6% perchloric acid and the derivatized captopril was extracted with diethyl ether. The chromatographic separation was performed on an analytical mu bondapak NH2 column (300 x 3.9 mm, i.d.) with an isocratic mobile phase consisting of n-hexane-2-propanol-methanol-acetic acid (68:15:15:2). Using ultraviolet detection at 246 nm, the quantification limit for captopril in plasma was 10 ng/ml. The calibration curve was linear over the concentration range 12.5-500 ng/ml. The average recovery was 95% for plasma. The inter-day and intra-day assay coefficients of variation were found to be less than 12%.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2-Bromo-2′-acetonaphthone, 99%

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