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Bright single-chain conjugated polymer dots embedded nanoparticles for long-term cell tracing and imaging.

Small (Weinheim an der Bergstrasse, Germany) (2013-12-18)
Guangxue Feng, Kai Li, Jie Liu, Dan Ding, Bin Liu
ABSTRACT

Single-chain conjugated polymer (CP) dots embedded nanoparticles (NPs) bearing cell penetration peptide (TAT) as surface ligands are synthesized for long term cancer cell tracing applications. The CPNPs are fabricated by matrix-encapsulation method and the embedded CPs can be modulated into spherical dots with different size upon alteration of feed concentrations. Single-chain CP dots are formed upon decreasing feed concentration to 0.2 mg/mL, where CPNPs exhibit highest fluorescence quantum yield of 32%. Maleimide is introduced as the new NP surface functional group, which favors easy conjugation with cell penetration peptide via click chemistry to preserve its biofunctions. The obtained CPNPs show high brightness and good biocompatibility, which allow cell tracing for over 9 generations, superior to commercial cell tracker Qtracker 585.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Maleimide, 99%