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Successful stabilization of functionalized hybrid graphene for high-performance antimicrobial activity.

Acta biomaterialia (2013-04-23)
Jeong A Nam, Abdullah-Al Nahain, Sung Min Kim, Insik In, Sung Young Park
ABSTRACT

We have prepared an antimicrobial nanocomposite composed of reduced graphene oxide (rGO) using antimicrobial agents and catechol derivative conjugated to polyethylene glycol-grafted poly(dimethylaminoethyl methacrylate) (PEG-g-PDMA). Graphene oxide (GO) has been simultaneously reduced by 2-chloro-3',4'-dihydroxyacetophenone (CCDP) in Tris buffer at pH 8.5 following catechol chemistry. Both CCDP and antimicrobial agent 1-bromododecane (C12) were quaternized to PEG-g-PDMA (CCDP-C12)-q-(PEG-g-PDMA). This synthesized polymer functionalized rGO as an antimicrobial nanocomposite, rGO/(CCDP-C12)-q-(PEG-g-PDMA). To increase antimicrobial activity, silver nanoparticles (Ag NPs) were deposited onto the high surface area of rGO/(CCDP-C12)-q-(PEG-g-PDMA). The prepared antimicrobial nanocomposite shows significant stability in aqueous media due to the hydrophilic behaviour of PEG. X-ray photoelectron spectroscopy investigation clearly shows the quaternization of C-12 and deposition of Ag NPs onto rGO surfaces. Ag NP-deposited rGO/(CCDP-C12)-q-(PEG-g-PDMA) shows better antimicrobial activity both against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria at lower concentration compared to without applying Ag NPs. Investigation of the cytotoxicity demonstrates outstanding non-toxic properties of both the prepared nanocomposite as well as the synthesized polymer.

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Sigma-Aldrich
1-Bromododecane, purum, ≥95.0% (GC)