• Home
  • Search Results
  • Determination of leakage from antibody adsorbent: composition analysis and pH effect.

Determination of leakage from antibody adsorbent: composition analysis and pH effect.

Biomedical chromatography : BMC (2013-05-18)
Lan Zhao, Yongdong Liu, Yinjue Wang, Yongdong Huang, Xiunan Li, Yan Li, Guanghui Ma, Zhiguo Su
ABSTRACT

To study the leakage at different solution pH values, IgG Sepharose 6FF®, a commercially available immunoadsorbent, was used as a model. The leaked substance consists of three parts: (1) ligands and its fragments; (2) ligands plus matrix fragments in which ligands are chemically attached to the adsorbent matrix; and (3) matrix fragments. Buffer solution pH values had a great effect on both the kinetics and the amount of ligand leakage. Cross-linking of the adsorbent matrix could reduce both matrix leakage and antibody leakage at pH 3.0, but its effect was limited at pH 11.0 for ligand leakage.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Agarose, BioReagent, for molecular biology, low EEO
Sigma-Aldrich
Agarose, Type I, low EEO
Sigma-Aldrich
Agarose, for molecular biology
Sigma-Aldrich
Sepharose® 4B, 45-165 μm bead diameter
Sigma-Aldrich
Agarose, Low EEO
Sigma-Aldrich
Agarose, Ultra-low Gelling Temperature, molecular biology grade
Sigma-Aldrich
Agarose, Ultra-low Gelling Temperature
Sigma-Aldrich
Agarose, Medium EEO, for molecular biology
Sigma-Aldrich
Agarose, Type I-A, low EEO
Sigma-Aldrich
Agarose, High Gelling Temperature
Sigma-Aldrich
Agarose, For pulsed field electrophoresis running gel
Sigma-Aldrich
Sepharose® 6B, 6% Beaded Agarose, 45-165 μm (wet), fractionation range 10,000-1,000,000 Da (dextrans)
Sigma-Aldrich
Agarose, Type II-A, Medium EEO
Sigma-Aldrich
Agarose, Wide range, for molecular biology
Sigma-Aldrich
Agarose, Low EEO, for Immunoelectrophoresis
Sigma-Aldrich
Agarose, High EEO, for molecular biology
Sigma-Aldrich
Agarose, High EEO
Sigma-Aldrich
Agarose, BioReagent, for molecular biology, Wide range/Standard 3:1
Sigma-Aldrich
Agarose, Type IV, Special High EEO
Sigma-Aldrich
Agarose, Special High EEO