MilliporeSigma
  • Home
  • Search Results
  • Four products from Escherichia coli pseudogenes increase hydrogen production.

Four products from Escherichia coli pseudogenes increase hydrogen production.

Biochemical and biophysical research communications (2013-09-13)
Mohd Zulkhairi Mohd Yusoff, Yuya Hashiguchi, Toshinari Maeda, Thomas K Wood
ABSTRACT

Pseudogenes are considered to be nonfunctional genes that lack a physiological role. By screening 3985 Escherichia coli mutants using chemochromic membranes, we found four pseudogenes involved in hydrogen metabolism. Knockouts of pseudogenes ydfW and ypdJ had a defective hydrogen phenotype on glucose and formate, respectively. Also, the knockout of pseudogene yqiG formed hydrogen from formate but not from glucose. For the yqiG mutant, 100% hydrogen recovery was obtained by the complementation of YqiG via a plasmid. The knockout of pseudogene ylcE showed hydrogen deficiency in minimal media which suggested that the role of YlcE is associated with cell growth. Hence, the products of these four pseudogenes play an important physiological role in hydrogen production in E. coli.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium formate, 99.998% trace metals basis
Sigma-Aldrich
Formic acid solution, BioUltra, 1.0 M in H2O
Sigma-Aldrich
Potassium formate, BioUltra, ≥99.0% (NT)
Sigma-Aldrich
Sodium formate, BioUltra, ≥99.0% (NT)
Sigma-Aldrich
Sodium formate-13C, 99 atom % 13C
Sigma-Aldrich
Potassium formate, ReagentPlus®, 99%
Supelco
Ammonium formate solution, 10 mM in H2O, suitable for HPLC
Sigma-Aldrich
Formic acid, ≥95%, FCC, FG
Sigma-Aldrich
Ammonium formate solution, BioUltra, 10 M in H2O
Sigma-Aldrich
Formic acid, ACS reagent, ≥96%
Sigma-Aldrich
Sodium formate, ACS reagent, ≥99.0%
Sigma-Aldrich
Formic acid, reagent grade, ≥95%
Sigma-Aldrich
Formic acid, puriss., meets analytical specifications of DAC, FCC, 98.0-100%
Sigma-Aldrich
Sodium formate, reagent grade, 97%
Sigma-Aldrich
Formic acid, ACS reagent, ≥88%
Sigma-Aldrich
Formic acid, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥98%
Supelco
Ammonium formate, eluent additive for LC-MS, LiChropur, ≥99.0%
Sigma-Aldrich
Calcium formate, BioUltra, ≥99.0% (T)
Sigma-Aldrich
Ammonium formate, BioUltra, ≥99.0% (calc. based on dry substance, NT)
Sigma-Aldrich
Thallium(I) formate, 97%
Sigma-Aldrich
Ammonium formate, ≥99.995% trace metals basis
Supelco
Calcium formate, Standard for quantitative NMR, TraceCERT®
Sigma-Aldrich
Cesium formate, 98%
Sigma-Aldrich
Basosiv M050, produced by BASF
Sigma-Aldrich
Ammonium formate, reagent grade, 97%