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Rhizobia survival in seeds coated with polyvinyl alcohol (PVA) electrospun nanofibres.

Canadian journal of microbiology (2013-11-12)
Raquel Damasceno, Isabel Roggia, Claudio Pereira, Enilson de Sá
ABSTRACT

The electrospinning technique of rhizobia immobilization in nanofibres is an innovative and promising alternative for reducing the harmful effects of environmental stress on bacteria strains in a possible inoculant nanotechnology product for use in agriculture. The use of polyvinyl alcohol (PVA) shows up as an effective polymer in cell encapsulation because of its physical characteristics, such as viscosity and power of scattering. The aim of these studies has been to evaluate the survival of rhizobia incorporated in PVA nanofibres, which were applied to soybean seed and then subjected to different storage times and exposure to fungicide. The maintenance of the symbiotic characteristics of the incorporated bacterial strains was also evaluated, noting the formation of nodules in the soybean seedlings. No significant differences in the cell survival at 0 h and after 24 h of storage were observed. After 48 h, a significant difference in the bacterial cell concentration of the seeds affixed with PVA nanofibres was observed. Exposure to the fungicide decreased the viability of the bacteria strains even when coated with the nanofibres. A larger number of nodules formed in soybean seedlings from seeds inoculated with rhizobia incorporated in PVA nanofibres than from seeds inoculated with rhizobia without PVA. Thus, the electrospinning technique is a great alternative to the usual protector inoculants because of its unprecedented capacity to control the release of bacteria.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Poly(vinyl alcohol), Mw 89,000-98,000, 99+% hydrolyzed
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Poly(vinyl alcohol), Mw 9,000-10,000, 80% hydrolyzed
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Poly(vinyl alcohol), 87-90% hydrolyzed, average mol wt 30,000-70,000
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Mowiol® 4-88, Mw ~31,000
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Poly(vinyl alcohol), Mw 13,000-23,000, 87-89% hydrolyzed
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Poly(vinyl alcohol), Mw 146,000-186,000, 99+% hydrolyzed
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Poly(vinyl alcohol), Mw 85,000-124,000, 99+% hydrolyzed
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Poly(vinyl alcohol), Mw 31,000-50,000, 98-99% hydrolyzed
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Poly(vinyl alcohol), average Mw 13,000-23,000, 98% hydrolyzed
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Poly(vinyl alcohol), average Mw 85,000-124,000, 87-89% hydrolyzed
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Poly(vinyl alcohol), Fully hydrolyzed
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Poly(vinyl alcohol), average Mw 31,000-50,000, 87-89% hydrolyzed
Sigma-Aldrich
Mowiol® 10-98, Mw ~61,000
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Mowiol® 18-88, Mw ~130,000
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Poly(vinyl alcohol), average Mw 146,000-186,000, 87-89% hydrolyzed
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Poly(vinyl alcohol), average Mw 130,000, 99+% hydrolyzed
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Mowiol® 40-88, average Mw ~205,000 g/mol
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Mowiol® 4-98, Mw ~27,000
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Mowiol® 8-88, Mw ~67,000
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Mowiol® 28-99, Mw ~145,000
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Mowiol® 20-98, Mw ~125,000
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Mowiol® 56-98, Mw ~195,000
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Mowiol® 6-98, Mw ~47,000