• Home
  • Search Results
  • Neuroestradiol in the hypothalamus contributes to the regulation of gonadotropin releasing hormone release.

Neuroestradiol in the hypothalamus contributes to the regulation of gonadotropin releasing hormone release.

The Journal of neuroscience : the official journal of the Society for Neuroscience (2013-12-07)
Brian P Kenealy, Amita Kapoor, Kathryn A Guerriero, Kim L Keen, James P Garcia, Joseph R Kurian, Toni E Ziegler, Ei Terasawa
ABSTRACT

Release of gonadotropin releasing hormone (GnRH) from the medial basal hypothalamus (MBH)/median eminence region (S-ME) is essential for normal reproductive function. GnRH release is profoundly regulated by the negative and positive feedback effects of ovarian estradiol (E2). Here we report that neuroestradiol, released in the S-ME, also directly influences GnRH release in ovariectomized female monkeys, in which the ovarian source of E2 is removed. We found that (1) brief infusion of E2 benzoate (EB) to the S-ME rapidly stimulated release of GnRH and E2 in the S-ME of ovariectomized monkeys, (2) electrical stimulation of the MBH resulted in GnRH release as well as E2 release, and (3) direct infusion of an aromatase inhibitor to the S-ME suppressed spontaneous GnRH release as well as the EB-induced release of GnRH and E2. These findings reveal the importance of neuroestradiol as a neurotransmitter in regulation of GnRH release. How circulating ovarian E2 interacts with hypothalamic neuroestrogens in the control of GnRH release remains to be investigated.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
β-Estradiol, ≥98%
Sigma-Aldrich
β-Estradiol, BioReagent, powder, suitable for cell culture
Sigma-Aldrich
Estradiol, meets USP testing specifications
Sigma-Aldrich
β-Estradiol, powder, γ-irradiated, suitable for cell culture
Supelco
17β-Estradiol solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®
Sigma-Aldrich
Letrozole, ≥98% (HPLC)
Sigma-Aldrich
β-Estradiol 3-benzoate, ≥97%
Sigma-Aldrich
β-Estradiol, analytical standard
Estradiol hemihydrate, European Pharmacopoeia (EP) Reference Standard
Letrozole, European Pharmacopoeia (EP) Reference Standard
Supelco
β-Estradiol 3-benzoate, VETRANAL®, analytical standard