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DNA damage in human lymphocytes exposed to four food additives in vitro.

Toxicology and industrial health (2012-11-29)
Serkan Yilmaz, Fatma Unal, Deniz Yüzbaşıoğlu, Mustafa Celik
ABSTRACT

In vitro genotoxic effects of antioxidant additives, such as citric acid (CA) and phosphoric acid (PA) and their combination, as well as antimicrobial additives, such as benzoic acid (BA) and calcium propionate (CP), on human lymphocytes were determined using alkaline single-cell gel electrophoresis. There was a significant increase in the DNA damage in human lymphocytes after 1 h of in vitro exposure to CA, PA, BA and CP (200, 25-200, 50-500, 50-1000 μg/mL, respectively). The combination of CA and PA significantly increased the mean tail intensity at all the concentrations used (25-200 μg/mL) and significantly increased the mean tail length mainly after higher concentrations (100 and 200 μg/mL). Data in this study showed that the concentrations of food additives used induce DNA damage and PA was the most genotoxic and CA was less genotoxic additives among them.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Calcium propionate, 99.0-100.5% (in dried substance), meets analytical specification of E 282