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Single-molecule fluorescence studies reveal long-range electron-transfer dynamics through double-stranded DNA.

Chemphyschem : a European journal of chemical physics and physical chemistry (2009-01-30)
Manoj Kumbhakar, Alexander Kiel, Haridas Pal, Dirk-Peter Herten
ABSTRACT

Complex dynamics of combined long-range fluorescence resonance energy transfer (FRET) and short-range electron transfer (ET) processes in a single double-stranded DNA (dsDNA) molecule (see figure) reveals that FRET remains almost unaltered in the presence of ET. Present systems also demonstrate the potential for long-range ET studies through the base stack in single dsDNA molecules.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Atto 520-Biotin, BioReagent, suitable for fluorescence, ≥95.0% (HPCE)
Supelco
Atto 520 NHS ester, BioReagent, suitable for fluorescence, ≥80.0% (coupling to amines)
Sigma-Aldrich
Atto 520 azide, suitable for fluorescence
Sigma-Aldrich
Atto 520 maleimide, for fluorescence, ≥90% (coupling rate)

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