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Sensitive bioanalysis: combining single-molecule spectroscopy with mono-labeled self-quenching probes.

Analytical and bioanalytical chemistry (2007-06-15)
Nicole Marmé, Jens-Peter Knemeyer
ABSTRACT

Fluorescence single-molecule spectroscopy is an appropriate tool for modern bioanalysis. This technique enables the development of ultra sensitive assays, especially when combined with self-quenching probes. In this review we report novel DNA, enzyme, and antibody assays based on mono-labeled fluorescent probes that are quenched by photoinduced electron transfer (PET).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Atto 655, BioReagent, suitable for fluorescence, ≥85% (HPLC)
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Atto 655 NHS ester, BioReagent, suitable for fluorescence
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Atto 655-Biotin, BioReagent, suitable for fluorescence, ≥95.0% (HPCE)
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Atto 680, BioReagent, suitable for fluorescence, ≥90% (HPLC)
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Atto 680 NHS ester, BioReagent, suitable for fluorescence, ≥90.0% (coupling to amines)
Sigma-Aldrich
Atto 655 amine, suitable for fluorescence
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Atto 680 maleimide, BioReagent, suitable for fluorescence, ≥90% (HPCE)
Sigma-Aldrich
Atto 655-maleimide, BioReagent, suitable for fluorescence, ≥90% (coupling rate)

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