Magnetic conjugated polymer nanoparticles as bimodal imaging agents.

Journal of the American Chemical Society (2010-06-25)
Philip Howes, Mark Green, Alex Bowers, David Parker, Gopal Varma, Mathew Kallumadil, Mary Hughes, Alice Warley, Anthony Brain, Rene Botnar
ABSTRACT

Hybrid nanoparticles which incorporate multiple functionalities, such as fluorescence and magnetism, can exhibit enhanced efficiency and versatility by performing several tasks in parallel. In this study, magnetic-fluorescent semiconductor polymer nanospheres (MF-SPNs) have been synthesized by encapsulation of hydrophobic conjugated polymers and iron oxide nanoparticles in phospholipid micelles. Four fluorescent conjugated polymers were used, yielding aqueous dispersions of nanoparticles which emit across the visible spectrum. The MF-SPNs were shown to be magnetically responsive and simultaneously fluorescent. In MRI studies, they were seen to have a shortening effect on the transverse T(2)* relaxation time, which demonstrates their potential as an MR contrast agent. Finally, successful uptake of the MF-SPNs by SH-SY5Y neuroblastoma cells was demonstrated, and they were seen to behave as bright and stable fluorescent markers. There was no evidence of toxicity or adverse affect on cell growth.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Iron oxide incorporated conjugated polymer nanoparticles, fluorescence λem 680 nm, 100 μg/mL in H2O
Sigma-Aldrich
Iron oxide incorporated conjugated polymer nanoparticles, fluorescence λem 475 nm, 100 μg/mL in H2O
Sigma-Aldrich
Iron oxide incorporated conjugated polymer nanoparticles, fluorescence λem 550 nm, 100 μg/mL in H2O
Sigma-Aldrich
Iron oxide incorporated conjugated polymer nanoparticles, fluorescence λem 420 nm, 100 μg/mL in H2O
Sigma-Aldrich
Iron oxide incorporated conjugated polymer nanoparticles, fluorescence λem 610 nm, 100 μg/mL in H2O
Sigma-Aldrich
Iron oxide incorporated conjugated polymer nanoparticles, fluorescence λem 435 nm, 100 μg/mL in H2O
Sigma-Aldrich
Iron oxide incorporated conjugated polymer nanoparticles, fluorescence λem 510 nm, 100 μg/mL in H2O
Sigma-Aldrich
Atto 390 azide, suitable for fluorescence
Supelco
Atto 390 NHS ester, BioReagent, suitable for fluorescence, ≥80% (coupling to amines)

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.