Single-pair FRET characterization of DNA tweezers.

Nano letters (2006-12-14)
Barbara K Müller, Andreas Reuter, Friedrich C Simmel, Don C Lamb
ABSTRACT

The self-assembly properties of DNA make it an ideal choice for the construction of nanomachines such as DNA tweezers. Because nanomachines function as individual units, they need to be studied on the single-molecule level. From single-pair FRET investigations, we show that "open" tweezers exist in a single conformation with minimal FRET efficiency, whereas upon addition of a "closing strand", three conformations are observed, which are averaged out in ensemble measurements.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Atto 647N NHS ester, BioReagent, suitable for fluorescence, ≥85% (coupling to amines)
Sigma-Aldrich
Atto 532 NHS ester, BioReagent, suitable for fluorescence, ≥80% (coupling to amines)
Supelco
Atto 647N, BioReagent, suitable for fluorescence, ≥98.0% (HPCE)
Supelco
Atto 647N maleimide, BioReagent, suitable for fluorescence, ≥90% (coupling to thiols)
Sigma-Aldrich
Atto 532, BioReagent, suitable for fluorescence, ≥90% (HPCE)
Sigma-Aldrich
Atto 647N-Biotin, BioReagent, ≥95% (HPCE), suitable for fluorescence
Sigma-Aldrich
Atto 647N azide
Sigma-Aldrich
Atto 532 maleimide, BioReagent, suitable for fluorescence, ≥90% (coupling to thiols)
Sigma-Aldrich
Atto 647N iodoacetamide
Sigma-Aldrich
Atto 647N amine

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