• Home
  • Search Results
  • Birefringent device converts a standard scanning microscope into a STED microscope that also maps molecular orientation.

Birefringent device converts a standard scanning microscope into a STED microscope that also maps molecular orientation.

Optics express (2010-02-23)
Matthias Reuss, Johann Engelhardt, Stefan W Hell
ABSTRACT

Stimulated emission depletion (STED) microscopy usually employs a scanning excitation beam that is superimposed by a donut-shaped STED beam for keeping the fluorophores at the periphery of the excitation spot dark. Here, we introduce a simple birefringent device that produces a donut-shaped focal spot with suitable polarization for STED, while leaving the excitation spot virtually intact. The device instantly converts a scanning (confocal) microscope with a co-aligned STED beam into a full-blown STED microscope. The donut can be adapted to reveal, through the resulting fluorescence image, the orientation of fluorophores in the sample, thus directly providing subdiffraction resolution images of molecular orientation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Atto 647N NHS ester, BioReagent, suitable for fluorescence, ≥85% (coupling to amines)
Sigma-Aldrich
Phalloidin–Atto 647N, BioReagent, suitable for fluorescence, ≥85% (HPCE)
Sigma-Aldrich
Atto 532 NHS ester, BioReagent, suitable for fluorescence, ≥80% (coupling to amines)
Supelco
Atto 565 NHS ester, BioReagent, suitable for fluorescence, ≥70.0% (coupling to amines)
Supelco
Atto 647N, BioReagent, suitable for fluorescence, ≥98.0% (HPCE)
Sigma-Aldrich
Atto 565, BioReagent, suitable for fluorescence, ≥75% (sum of isomers, HPCE/HPLC)
Sigma-Aldrich
Anti-Rabbit-IgG - Atto 647N antibody produced in goat, 1 mg/mL IgG
Sigma-Aldrich
Anti-Mouse-IgG - Atto 647N antibody produced in goat, contains 50% glycerol as stabilizer
Sigma-Aldrich
Atto 425 NHS ester, BioReagent, suitable for fluorescence
Supelco
Atto 647N maleimide, BioReagent, suitable for fluorescence, ≥90% (coupling to thiols)
Sigma-Aldrich
Atto 647 NHS ester, BioReagent, suitable for fluorescence, ≥80% (coupling to amines)
Sigma-Aldrich
Atto 565-Biotin, BioReagent, suitable for fluorescence, ≥90% (HPCE)
Sigma-Aldrich
Atto 647N DOPE, suitable for fluorescence
Sigma-Aldrich
Atto 647N-Streptavidin, BioReagent, suitable for fluorescence
Sigma-Aldrich
Atto 647N Protein Labeling Kit
Sigma-Aldrich
Atto 425
Supelco
NTA - Atto 647 N, BioReagent, suitable for fluorescence, ≥80% (HPCE)
Sigma-Aldrich
Atto 532, BioReagent, suitable for fluorescence, ≥90% (HPCE)
SAFC
Atto 465 NHS ester, BioReagent, suitable for fluorescence, ≥80% (HPCE)
Sigma-Aldrich
Atto 590-Biotin, BioReagent, suitable for fluorescence, ≥90.0% (HPCE)
Sigma-Aldrich
Phalloidin–Atto 390, suitable for fluorescence, ≥95.0% (HPCE)
Supelco
Atto 590 NHS ester, BioReagent, suitable for fluorescence, ≥60% (coupling to amines)
Sigma-Aldrich
Atto 425-Biotin, BioReagent, suitable for fluorescence, ≥95.0% (HPCE)
Sigma-Aldrich
Atto 647N-Biotin, BioReagent, ≥95% (HPCE), suitable for fluorescence
Sigma-Aldrich
Atto 565 maleimide, BioReagent, suitable for fluorescence
Sigma-Aldrich
Atto 425-Streptavidin, BioReagent, suitable for fluorescence, ≥95% (HPCE)
Sigma-Aldrich
Atto 647N azide
Sigma-Aldrich
Phalloidin–Atto 532, suitable for fluorescence, ≥95.0% (HPCE)
Sigma-Aldrich
Atto 532 maleimide, BioReagent, suitable for fluorescence, ≥90% (coupling to thiols)
Sigma-Aldrich
Atto 532 DOPE, suitable for fluorescence

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.