• Home
  • Search Results
  • Impact of tumor cell cytoskeleton organization on invasiveness and migration: a microchannel-based approach.

Impact of tumor cell cytoskeleton organization on invasiveness and migration: a microchannel-based approach.

PloS one (2010-01-22)
Claudio G Rolli, Thomas Seufferlein, Ralf Kemkemer, Joachim P Spatz

Cell migration is a fundamental feature of the interaction of cells with their surrounding. The cell's stiffness and ability to deform itself are two major characteristics that rule migration behavior especially in three-dimensional tissue. We simulate this situation making use of a micro-fabricated migration chip to test the active invasive behavior of pancreatic cancer cells (Panc-1) into narrow channels. At a channel width of 7 microm cell migration through the channels was significantly impeded due to size exclusion. A striking increase in cell invasiveness was observed once the cells were treated with the bioactive lipid sphingosylphosphorylcholine (SPC) that leads to a reorganization of the cell's keratin network, an enhancement of the cell's deformability, and also an increase in the cell's migration speed on flat surfaces. The migration speed of the highly deformed cells inside the channels was three times higher than of cells on flat substrates but was not affected upon SPC treatment. Cells inside the channels migrated predominantly by smooth sliding while maintaining constant cell length. In contrast, cells on adhesion mediating narrow lines moved in a stepwise way, characterized by fluctuations in cell length. Taken together, with our migration chip we demonstrate that the dimensionality of the environment strongly affects the migration phenotype and we suggest that the spatial cytoskeletal keratin organization correlates with the tumor cell's invasive potential.

Product Number
Product Description

Atto 488 NHS ester, BioReagent, suitable for fluorescence, ≥80% (coupling to amines)
Atto 488, suitable for fluorescence, ≥90% (HPCE)
Atto 488-Biotin, BioReagent, suitable for fluorescence, ~75% (HPCE)
Atto 488 maleimide, BioReagent, suitable for fluorescence, ≥90% (coupling to thiols)
Atto 488 amine
Atto 488 azide
Atto 488 iodoacetamide

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon


Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.