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The effect of low-frequency ultrasound on the activity and efficiency of a commercial cellulase enzyme.

Carbohydrate polymers (2013-09-24)
Orsolya Erzsébet Szabó, Emília Csiszár
ABSTRACT

A commercial acidic cellulase enzyme complex was chosen in order to gain detailed information about the effect of low-frequency ultrasound (horn at 40 kHz) on the enzyme activity. The performance of the enzyme under sonication was also evaluated in a cellulose-cellulase model reaction. The filter paper activity of the enzyme and the yield of the enzyme catalysed hydrolysis were determined as a function of the parameters of the sonicated environment (treatment time, amplitude, with and without a reflector) and compared with the data measured in a non-sonicated bath. Depending on the parameters of the sonication, the enzyme is susceptible to ultrasound and its activity can significantly decrease. Despite the serious reduction of the enzyme activity, the outcome of the enzyme catalysed hydrolysis was always positive, implying that the advantageous effects of sonication impressed on the heterogeneous enzyme reaction always overcome the undesirable enzyme modifying effect of ultrasound.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Cellulase from Trichoderma reesei, aqueous solution, ≥700 units/g
Sigma-Aldrich
Cellulase from Aspergillus niger, powder, ≥0.3 units/mg solid
Sigma-Aldrich
Cellulase from Trichoderma reesei ATCC 26921, lyophilized powder, ≥1 unit/mg solid
Sigma-Aldrich
Cellulase from Aspergillus sp., aqueous solution
Sigma-Aldrich
Cellulase from Trichoderma sp., BioReagent, suitable for plant cell culture, 3-10 units/mg solid
Sigma-Aldrich
Cellulase from Aspergillus niger, powder, off-white, ~0.8 U/mg
Sigma-Aldrich
Cellulase from Trichoderma sp., powder, ≥5,000 units/g solid

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