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Urease inhibitory constituents from Daphne retusa.

Journal of Asian natural products research (2013-11-26)
Farrukh Mansoor, Itrat Anis, Ajmal Khan, Bishnu P Marasini, Muhammad Iqbal Choudhary, Muhammad Raza Shah
ABSTRACT

The bioassay-guided fractionation of Daphne retusa Hemsl. has led to the isolation of a new aryl tetrahydronaphthalene lignan derivative named as daphnretusic acid (1), along with six new source compounds such as 5,7-dihydroxyflavone (2), 7-hydroxyflavone (3), 6-methoxyflavone (4), (+) pinoresinol (5), (+) sesamin (6), and β-sitosterol-3-O-β-D-glucopyranoside (7). Their structures were elucidated by (1)H NMR, (13)C NMR, 1D, 2D NMR, UV, IR, and EIMS analyses. All the fractions (n-hexane, CHCl3, AcOEt, CH3OH, and water) and pure compounds (1-7) were subjected to the assay of urease and α-chymotrypsin inhibitory activities. Chloroform and methanol soluble fractions showed moderate urease inhibition. Compound 2 exhibited significant urease inhibition with IC50 value 60.4 ± 0.72 μM, whereas compounds 1 and 3-7 remained inactive during urease inhibition and α-chymotrypsin bioassays.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
α-Chymotrypsin from bovine pancreas, (TLCK treated to inactivate residual tryspin activity), Type VII, essentially salt-free, lyophilized powder, ≥40 units/mg protein
Sigma-Aldrich
Urease from Canavalia ensiformis (Jack bean), Type C-3, powder, ≥600,000 units/g solid
Sigma-Aldrich
Urease from Canavalia ensiformis (Jack bean), Type III, glycerol solution, 500-800 units/mL
Sigma-Aldrich
Urease from Canavalia ensiformis (Jack bean), Type III, powder, 15,000-50,000 units/g solid
Sigma-Aldrich
Urease from Canavalia ensiformis (Jack bean)
Sigma-Aldrich
α-Chymotrypsin from bovine pancreas, Type I-S, essentially salt-free, lyophilized powder
Sigma-Aldrich
α-Chymotrypsin from bovine pancreas, ≥40 units/mg protein, vial of 5 mg
Sigma-Aldrich
α-Chymotrypsin from human pancreas, lyophilized powder
Sigma-Aldrich
Urease from Canavalia ensiformis (Jack bean), Type IX, powder, 50,000-100,000 units/g solid
Sigma-Aldrich
α-Chymotrypsin from bovine pancreas, Type II, lyophilized powder, ≥40 units/mg protein
Sigma-Aldrich
α-Chymotrypsin from bovine pancreas, suitable for protein sequencing, salt-free, lyophilized powder
Sigma-Aldrich
Urease from Canavalia ensiformis (Jack bean), powder, white, ~8 U/mg
Sigma-Aldrich
Urease from Canavalia ensiformis (Jack bean), powder, ~1 U/mg
Sigma-Aldrich
α-Chymotrypsin−Agarose from bovine pancreas, lyophilized powder, 2,000-3,500 units/g agarose (One ml gel will yield 65-120 units)