MilliporeSigma

Fractionated marine invertebrate extract libraries for drug discovery.

Molecules (Basel, Switzerland) (2008-07-04)
Tim S Bugni, Mary Kay Harper, Malcolm W B McCulloch, Jason Reppart, Chris M Ireland
ABSTRACT

The high-throughput screening and drug discovery paradigm has necessitated a change in preparation of natural product samples for screening programs. In an attempt to improve the quality of marine natural products samples for screening, several fractionation strategies were investigated. The final method used HP20SS as a solid support to effectively desalt extracts and fractionate the organic components. Additionally, methods to integrate an automated LCMS fractionation approach to shorten discovery time lines have been implemented.

MATERIALS
Product Number
Brand
Product Description

Supelco
Diaion® HP-20SS, jar of 100 g
Supelco
Diaion® HP-20SS, bucket of 1000 g