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Molecular mechanisms of boron transport in plants: involvement of Arabidopsis NIP5;1 and NIP6;1.

Advances in experimental medicine and biology (2010-07-30)
Kyoko Miwa, Mayuki Tanaka, Takehiro Kamiya, Toru Fujiwara
ABSTRACT

Understanding of the molecular mechanisms of boron (B) transport has been greatly advanced in the last decade. BOR1, the first B transporter in living systems, was identified by forward genetics using Arabidopsis mutants. Genes similar to BOR1 have been reported to share different physiological roles in plants. NIPS;1, a member of aquaporins in Arabidopsis, was then identified as a boric acid channel gene responsible for the B uptake into roots. NIP6;1, the most similar gene to NIPS;1, encodes a B channel essential for B distribution to young leaves. In the present chapter, recent advancement of the understanding of molecular mechanisms of B transport and roles of NIP genes are discussed.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Boron, ≥95% (boron), amorphous powder
Sigma-Aldrich
Boron, crystalline, −60 mesh, 99% trace metals basis
Sigma-Aldrich
Boron, crystalline, 1 cm, 99.7% trace metals basis
Boron, monofilament, 5m, diameter 0.1mm
Boron, monofilament, 20m, diameter 0.2mm
Boron, monofilament, 50m, diameter 0.1mm
Boron, monofilament, 200m, diameter 0.1mm
Boron, microfoil, disks, 25mm, thinness 0.1μm, specific density 23.5μg/cm2, permanent mylar 3.5μm support, 99.6%
Boron, monofilament, 20m, diameter 0.1mm
Boron, monofilament, 5m, diameter 0.2mm
Boron, monofilament, 100m, diameter 0.1mm
Boron, monofilament, 10m, diameter 0.1mm
Boron, microfoil, disks, 10mm, thinness 0.1μm, specific density 23.5μg/cm2, permanent mylar 3.5μm support, 99.6%
Boron, monofilament, 10m, diameter 0.2mm
Boron, monofilament, 50m, diameter 0.2mm