Mycotoxin biosynthesis is affected by peculiar agro-ecosystem dependent conditions. Accordingly, the correlation between mycotoxin contamination and environmental condition change scenarios should be carefully considered. This is because fungal colonization and mycotoxin contamination could critically impair the availability of food products, especially in poor countries. At any time, the agricultural products might become susceptible to fungal contamination and mycotoxin production in uncontrolled storage. From 2000 to 2010, many studies were carried out in the case of the effects of different parameters on ochratoxin A (OTA) production by fungi in order to avoid providing the appropriate conditions for producing OTA. Water activity (a(w)), temperature, type of species and the substrates were introduced as the most important in these articles. This paper reviews the major published articles regarding the different ecophysiological factors influencing on OTA production.