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Hydrogen/deuterium exchange mass spectrometry for probing higher order structure of protein therapeutics: methodology and applications.

Drug discovery today (2013-08-10)
Hui Wei, Jingjie Mo, Li Tao, Reb J Russell, Adrienne A Tymiak, Guodong Chen, Roxana E Iacob, John R Engen
ABSTRACT

The higher order structure of protein therapeutics can be interrogated with hydrogen/deuterium exchange mass spectrometry (HDX-MS). HDX-MS is now a widely used tool in the structural characterization of protein therapeutics. In this review, HDX-MS based workflows designed for protein therapeutic discovery and development processes are presented, focusing on the specific applications of epitope mapping for protein/drug interactions and biopharmaceutical comparability studies. Future trends in the application of HDX-MS in protein therapeutics characterization are also described.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Hydrogen, ≥99.99%
Sigma-Aldrich
Deuterium, 99.8 atom % D
Sigma-Aldrich
Deuterium, 99.96 atom % D
Sigma-Aldrich
Deuterium, 99.9 atom % D