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  • Pseudomonas fluorescens JH 70-4 promotes pb stabilization and early seedling growth of sudan grass in contaminated mining site soil.

Pseudomonas fluorescens JH 70-4 promotes pb stabilization and early seedling growth of sudan grass in contaminated mining site soil.

Environmental technology (2014-08-26)
Jaehong Shim, A Giridhar Babu, Palanivel Velmurugan, Patrick J Shea, Byung-Taek Oh
ABSTRACT

A bacterial strain (JH 70-4) exhibiting plant growth promoting characteristics (indoleacetic acid production and 1-aminocyclopropane-1-carboxylate deaminase activity), as well as heavy metal(loid) (HM) tolerance and Pb precipitation, was isolated from HM-contaminated soil at an abandoned mine site. The bacterium was identified as Pseudomonas fluorescens based on 16S rDNA sequencing. The JH 70-4 strain induced precipitation of Pb as PbS nanoparticles, confirmed by X-ray diffraction. Solution pH, incubation time, and Pb concentration influenced removal and PbS formation. Inoculating contaminated soil with JH 70-4 decreased Pb availability; exchangeable Pb decreased while organic- and sulphide-bound Pb increased. The toxicity characteristic leaching procedure showed a 65% decrease in Pb in leachate 60 d after inoculating soil with JH 70-4. Shoot and root lengths of Sudan grass grown in the inoculated soil were greater than in the uninoculated soil. Findings suggest that microbial Pb fixation is a viable strategy for remediating soil and promoting plant growth for phytostabilization of contaminated sites.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Lead, powder, −100 mesh, 99.95% trace metals basis
Sigma-Aldrich
Lead, shot, <2 mm, 99.9% trace metals basis
Sigma-Aldrich
Lead, shot, 1-3 mm, 99.995% trace metals basis
Lead, foil, 100x100mm, thickness 1.5mm, as rolled, 99.99+%
Lead, foil, light tested, 100x100mm, thickness 0.025mm, 99.99+%
Lead, foil, 25x25mm, thickness 3.0mm, as rolled, 99.99+%
Lead, foil, 1m coil, thickness 0.016mm, as rolled, 96% (sn 1.5%, sb 2.5%)
Lead, foil, 15mm disks, thickness 0.009mm, 99.99+%
Lead, foil, 0.5m coil, thickness 0.016mm, as rolled, 96% (sn 1.5%, sb 2.5%)
Lead, foil, 25x25mm, thickness 0.125mm, as rolled, 99.99+%
Lead, foil, 10m coil, thickness 0.12mm, coil width 25.4mm, as rolled, 99.95%
Lead, foil, 15mm disks, thickness 0.0125mm, 99.99+%
Lead, foil, 1m coil, thickness 0.15mm, as rolled, 99.95%
Lead, foil, 10mm disks, thickness 0.1mm, as rolled, 99.95%
Lead, foil, 25mm disks, thickness 0.05mm, 99.99+%
Lead, foil, 25x25mm, thickness 1.0mm, as rolled, 99.95%
Lead, foil, 4mm disks, thickness 0.125mm, as rolled, 99.95%
Lead, foil, 8mm disks, thickness 0.01mm, 99.99+%
Lead, foil, 4mm disks, thickness 0.25mm, as rolled, 99.95%
Lead, foil, 100x100mm, thickness 0.25mm, as rolled, 99.99+%
Lead, foil, 8mm disks, thickness 0.25mm, as rolled, 99.95%
Lead, foil, 50mm disks, thickness 0.1mm, as rolled, 99.95%
Lead, foil, 300x300mm, thickness 0.5mm, as rolled, 99.95%
Lead, foil, 5m coil, thickness 0.15mm, as rolled, 99.95%
Lead, foil, 50x50mm, thickness 0.25mm, as rolled, 99.95%
Lead, foil, 6mm disks, thickness 0.125mm, as rolled, 99.95%
Lead, foil, 25x25mm, thickness 0.55mm, as rolled, 99.999%
Lead, foil, 100x100mm, thickness 6.0mm, as rolled, 99.99+%
Lead, foil, 50x50mm, thickness 1.5mm, as rolled, 99.99+%
Lead, foil, 5m coil, thickness 0.05mm, as rolled, 97% (sn 1.5%, sb 1.5%)