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Increased drought tolerance through the suppression of ESKMO1 gene and overexpression of CBF-related genes in Arabidopsis.

PloS one (2014-09-04)
Fuhui Xu, Zhixue Liu, Hongyan Xie, Jian Zhu, Juren Zhang, Josef Kraus, Tasja Blaschnig, Reinhard Nehls, Hong Wang
ABSTRACT

Improved drought tolerance is always a highly desired trait for agricultural plants. Significantly increased drought tolerance in Arabidopsis thaliana (Columbia-0) has been achieved in our work through the suppression of ESKMO1 (ESK1) gene expression with small-interfering RNA (siRNA) and overexpression of CBF genes with constitutive gene expression. ESK1 has been identified as a gene linked to normal development of the plant vascular system, which is assumed directly related to plant drought response. By using siRNA that specifically targets ESK1, the gene expression has been reduced and drought tolerance of the plant has been enhanced dramatically in the work. However, the plant response to external abscisic acid application has not been changed. ICE1, CBF1, and CBF3 are genes involved in a well-characterized plant stress response pathway, overexpression of them in the plant has demonstrated capable to increase drought tolerance. By overexpression of these genes combining together with suppression of ESK1 gene, the significant increase of plant drought tolerance has been achieved in comparison to single gene manipulation, although the effect is not in an additive way. Accompanying the increase of drought tolerance via suppression of ESK1 gene expression, the negative effect has been observed in seeds yield of transgenic plants in normal watering conditions comparing with wide type plant.

MATERIALS
Product Number
Brand
Product Description

MISSION® siRNA Product Offerings, Custom and Predesigned siRNA
Sigma-Aldrich
Kanamycin sulfate, mixture of Kanamycin A (main component) and Kanamycin B and C
Sigma-Aldrich
Spectrum Plant Total RNA Kit, sufficient for 50 purifications
Sigma-Aldrich
Spectrum Plant Total RNA Kit, sufficient for 250 purifications
Sigma-Aldrich
Murashige and Skoog Basal Medium, suitable for plant cell culture, with sucrose and agar
Sigma-Aldrich
GenElute Plant Genomic DNA Miniprep Kit, sufficient for 70 purifications
Sigma-Aldrich
GenElute Plant Genomic DNA Miniprep Kit, sufficient for 350 purifications
Sigma-Aldrich
Spectrum Plant Total RNA Kit, sufficient for 10 purifications
Sigma-Aldrich
GenElute Plant Genomic DNA Miniprep Kit, sufficient for 10 purifications