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  • DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a complex at replication foci.

DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a complex at replication foci.

Nature genetics (2000-07-11)
M R Rountree, K E Bachman, S B Baylin
ABSTRACT

DNA methylation can contribute to transcriptional silencing through several transcriptionally repressive complexes, which include methyl-CpG binding domain proteins (MBDs) and histone deacetylases (HDACs). We show here that the chief enzyme that maintains mammalian DNA methylation, DNMT1, can also establish a repressive transcription complex. The non-catalytic amino terminus of DNMT1 binds to HDAC2 and a new protein, DMAP1 (for DNMT1 associated protein), and can mediate transcriptional repression. DMAP1 has intrinsic transcription repressive activity, and binds to the transcriptional co-repressor TSG101. DMAP1 is targeted to replication foci through interaction with the far N terminus of DNMT1 throughout S phase, whereas HDAC2 joins DNMT1 and DMAP1 only during late S phase, providing a platform for how histones may become deacetylated in heterochromatin following replication. Thus, DNMT1 not only maintains DNA methylation, but also may directly target, in a heritable manner, transcriptionally repressive chromatin to the genome during DNA replication.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-DNMT1 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Anti-DNMT1 antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution