F-box proteins: the key to protein degradation.

Journal of biomedical science (2006-02-08)
Margaret S Ho, Pei-I Tsai, Cheng-Ting Chien
ABSTRACT

The eukaryotic protein degradation pathway involves the ubiquitin (Ub) modification of substrates targeted for degradation by the 26S proteasome. The addition of Ub, a process called ubiquitination, is mediated by enzymes including the E3 Ub ligases which transfer the Ub to targeted substrates. A major type of E3 Ub ligases, the SCF (Skp-Cullin-F-box) complex, is composed of four major components: Skp1, Cul1/Cdc53, Roc1/Rbx1/Hrt1, and an F-box protein. The F-box component of the SCF machineries is responsible for recognizing different substrates for ubiquitination. Interaction with components of the SCF complex is mediated through the F-box motif of the F-box protein while it associates with phosphorylated substrates through its second protein-protein interaction motif such as Trp-Asp (WD) repeats or leucine-rich repeats (LRRs). By targeting diverse substrates, F-box proteins exert controls over stability of proteins and regulate the mechanisms for a wide-range of cellular processes. Here we discuss the importance of F-box proteins by providing a general overview and examples of how F-box proteins function in various cellular settings such as tissue development, cell proliferation, and cell death, in the modeling organism Drosophila.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-hFBXW7 antibody , Mouse monoclonal, clone FB407, purified from hybridoma cell culture
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Anti-FBG3 (FBXO44) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
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Anti-FBG4 (FBXO17) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

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