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Annexin A3 plays a role in cytoplasmic calcium oscillation by extracellular calcium in the human promyelocytic leukemia HL-60 cells differentiated by phorbol-12-myristate-13-acetate.

Experimental and molecular pathology (2014-07-19)
Hiroshi Nishiura, Koji Yamanegi, Mutsuki Kawabe, Nahoko Kato-Kogoe, Naoko Yamada, Keiji Nakasho
ABSTRACT

The roles of annexin A3 (ANXA3) in macrophages are not fully understood. In contrast to C5a, we have demonstrated that C-terminal ribosomal protein S19 (RP S19)-tagged S-tagged C5a (S-tagged C5a/RP S19) raises an alternative cytoplasmic calcium oscillation by extracellular calcium during macrophage migration into apoptotic cells. We here differentiated human promyelocytic leukemia HL-60 cells bearing with either control sense RNA and shRNA for ANXA3 mRNA or a vector cDNA with or without ANXA3 cDNA into macrophage-like cells by phorbol-12-myristate-13-acetate and found that a fluorescence ratio (340 nm/380 nm) upon the S-tagged C5a/RP S19-induced alternative cytoplasmic calcium oscillation by extracellular calcium was an equilateral association with a dose of ANXA3. Moreover, the ANXA3-dependent modification was partially reflected upon the S-tagged C5a-induced classical cytoplasmic calcium oscillation by both intracellular calcium and extracellular calcium. ANXA3 seems to extend the C5aR-mediated cytoplasmic calcium oscillation by extracellular calcium at least in the HL-60 macrophage-like cells.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Phorbol 12-myristate 13-acetate, ≥99% (TLC), film or powder
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PMA, for use in molecular biology applications, ≥99% (HPLC)
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Phorbol 12-myristate 13-acetate, synthetic, ≥98.0% (TLC)
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4α-Phorbol 12-myristate 13-acetate, solid, ≥95% (TLC)
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Anti-ANXA3 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
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Anti-MAPK14 (Ab-182) antibody produced in rabbit, affinity isolated antibody
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Anti-ANXA3 (AB2) antibody produced in rabbit, IgG fraction of antiserum
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Anti-ANXA3 (AB1) antibody produced in rabbit, IgG fraction of antiserum
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Anti-MAPK14 antibody produced in rabbit, affinity isolated antibody
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Anti-ANXA3 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
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Anti-MAP Kinase 2 (ERK-2) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution, ~1 mg/mL
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Anti-p38 MAP Kinase antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

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