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  • Effect of lead on phytotoxicity, growth, biochemical alterations and its role on genomic template stability in Sesbania grandiflora: a potential plant for phytoremediation.

Effect of lead on phytotoxicity, growth, biochemical alterations and its role on genomic template stability in Sesbania grandiflora: a potential plant for phytoremediation.

Ecotoxicology and environmental safety (2014-08-12)
Srinivasan Malar, Rajendiran Manikandan, Paulo J C Favas, Shivendra Vikram Sahi, Perumal Venkatachalam
ABSTRACT

The present study was aimed at evaluating phytotoxicity of various concentrations of lead nitrate (0, 100, 200, 400, 600, 800 and 1000mgL(-1)) in Sesbania grandiflora. The seedling growth was significantly affected (46%) at 1000mgL(-1) lead (Pb) treatment. Accumulation of Pb content was high in root (118mgg(-1) dry weight) than in shoot (23mgg(-1) dry weight). The level of photosynthetic pigment contents was gradually increased with increasing concentrations of Pb. Malondialdehyde (MDA) content increased in both the leaves as well as roots at 600mgL(-1) Pb treatment and decreased at higher concentrations. The activity of antioxidative enzymes such as superoxide dismutase and peroxidase were positively correlated with Pb treatment while catalase and ascorbate peroxidase activities increased up to 600mgL(-1) Pb treatment and then slightly decreased at higher concentrations. Isozyme banding pattern revealed the appearance of additional isoforms of superoxide dismutase and peroxidase in Pb treated leaf tissues. Isozyme band intensity was more consistent with the respective changes in antioxidative enzyme activities. Random amplified polymorphic DNA results indicated that genomic template stability (GTS) was significantly affected based on Pb concentrations. The present results suggest that higher concentrations of Pb enhanced the oxidative damage by over production of ROS in S. grandiflora that had potential tolerance mechanism to Pb as evidenced by increased level of photosynthetic pigments, MDA content, and the level of antioxidative enzymes. Retention of high levels of Pb in root indicated that S. grandiflora has potential for phytoextracting heavy metals by rhizofiltration.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Lead, powder, −325 mesh, ≥99% trace metals basis
Sigma-Aldrich
Lead, wire, diam. 1.0 mm, 99.99% trace metals basis
Supelco
Lead (impurities), manufactured by MBH Analytical Ltd (83X PR8)
Lead, foil, 25mm disks, thickness 0.125mm, as rolled, 99.95%
Lead, foil, not light tested, 100x100mm, thickness 0.007mm, 99.99+%
Lead, foil, not light tested, 100x100mm, thickness 0.009mm, 99.99+%
Lead, foil, light tested, 50x50mm, thickness 0.025mm, 99.99+%
Lead, foil, not light tested, 150x150mm, thickness 0.03mm, 99.99+%
Lead, foil, not light tested, 100x100mm, thickness 0.03mm, 99.99+%
Lead, foil, not light tested, 100x100mm, thickness 0.006mm, 99.99+%
Lead, foil, 50x50mm, thickness 1.0mm, as rolled, 99.95%
Lead, foil, 25x25mm, thickness 0.25mm, as rolled, 99.99+%
Lead, foil, 50x50mm, thickness 1.0mm, as rolled, 99.99+%
Lead, foil, 6mm disks, thickness 0.5mm, as rolled, 99.95%
Lead, foil, 25x25mm, thickness 1.0mm, as rolled, 99.5%
Lead, foil, 50x50mm, thickness 3.0mm, as rolled, 99.95%
Lead, foil, 300x300mm, thickness 1.0mm, as rolled, 99.95%
Lead, foil, 100x100mm, thickness 3.0mm, as rolled, 99.99+%
Lead, foil, 6mm disks, thickness 0.006mm, 99.99+%
Lead, foil, 6mm disks, thickness 0.04mm, 99.99+%
Lead, foil, 25x25mm, thickness 0.125mm, as rolled, 99.95%
Lead, foil, 6mm disks, thickness 0.5mm, as rolled, 99.99+%
Lead, foil, 25x25mm, thickness 0.5mm, as rolled, 99.99+%
Lead, foil, 50x50mm, thickness 2.7mm, as rolled, 99.95%
Lead, foil, 50x50mm, thickness 3.0mm, as rolled, 99.99+%
Lead, foil, 8mm disks, thickness 0.008mm, 99.99+%
Lead, foil, 8mm disks, thickness 0.02mm, 99.99+%
Lead, foil, 300x300mm, thickness 0.25mm, as rolled, 99.95%
Lead, foil, 50mm disks, thickness 0.125mm, as rolled, 99.95%
Lead, foil, 8mm disks, thickness 0.55mm, as rolled, 100%