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Using the hemoglobin content of reticulocytes (RET-He) to evaluate anemia in patients with cancer.

American journal of clinical pathology (2014-09-23)
Ellinor I B Peerschke, Melissa S Pessin, Peter Maslak
ABSTRACT

Evaluation of anemia, particularly iron deficiency, in patients with cancer is difficult. This study examined using the hemoglobin content of reticulocytes (RET-He) to rule out iron deficiency, as defined by serum iron studies (transferrin saturation <20%, serum iron <40 μg/dL, and ferritin <100 ng/mL), in an unselected cancer patient population. Patients were entered into the study based on the existence of concurrent laboratory test requests for CBC and serum iron studies. Using a threshold of 32 pg/cell, RET-He ruled out iron deficiency with a negative predictive value (NPV) of 98.5% and 100%, respectively, in the study population (n = 209) and in a subpopulation of patients with low reticulocyte counts (n = 19). In comparison, the NPV of traditional CBC parameters (hemoglobin, <11 g/dL; mean corpuscular volume, <80 fL) was only 88.5%. These results support the use of RET-He in the evaluation of iron deficiency in a cancer care setting.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Ferritin from equine spleen, Type I, saline solution
Sigma-Aldrich
Iron, chips, 99.98% trace metals basis
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Ferritin from human spleen, Type V, 10 μg/mL in 0.15 M NaCl, 10 mM Tris, pH 8.0, containing 0.1% sodium azide
Sigma-Aldrich
Iron, foil, thickness 0.1 mm, ≥99.9% trace metals basis
Sigma-Aldrich
Iron, wire, diam. 0.5 mm, ≥99.9% trace metals basis
Sigma-Aldrich
Iron, powder, −325 mesh, 97%
Sigma-Aldrich
Iron, rod, diam. 6.3 mm, 99.98% trace metals basis
Sigma-Aldrich
Iron, puriss. p.a., carbonyl-Iron powder, low in magnesium and manganese compounds, ≥99.5% (RT)
Iron, IRMM®, certified reference material, 0.5 mm wire
Iron, rod, 1000mm, diameter 25mm, as drawn, 98+%
Iron, foil, 150x150mm, thickness 0.125mm, hard, 99.5%
Iron, foil, not light tested, 50x50mm, thickness 0.009mm, 99.85%
Iron, rod, 200mm, diameter 50mm, as drawn, armcO« soft ingot 99.8%
Iron, foil, 50x50mm, thickness 0.25mm, as rolled, 99.99+%
Iron, tube, 200mm, outside diameter 12.0mm, inside diameter 9mm, wall thickness 1.5mm, annealed, 99.5%
Iron, foil, not light tested, 50x50mm, thickness 0.0025mm, 99.85%
Iron, foil, 300x300mm, thickness 1.0mm, as rolled, armco« soft ingot 99.8%
Iron, tube, 100mm, outside diameter 6.35mm, inside diameter 5.35mm, wall thickness 0.5mm, as drawn, 99.8%
Iron, rod, 50mm, diameter 12.7mm, as drawn, 99.95%
Iron, rod, 100mm, diameter 5.0mm, as drawn, 99.99+%
Iron, tube, 100mm, outside diameter 1.0mm, inside diameter 0.8mm, wall thickness 0.1mm, as drawn, 99.5%
Iron, foil, 25x25mm, thickness 0.125mm, as rolled, 99.99+%
Iron, rod, 500mm, diameter 19mm, as drawn, soft ingot 99.8%
Iron, rod, 50mm, diameter 40mm, as drawn, armcO« soft ingot 99.8%
Iron, tube, 200mm, outside diameter 6.35mm, inside diameter 5.35mm, wall thickness 0.5mm, as drawn, 99.8%
Iron, foil, 10mm disks, thickness 0.008mm, 99.85%
Iron, foil, 0.5m coil, thickness 0.038mm, hard, 99.5%
Iron, foil, 300x300mm, thickness 0.125mm, hard, 99.5%
Iron, foil, 25x25mm, thickness 0.9mm, as rolled, 99.5%
Iron, foil, 150x150mm, thickness 0.5mm, hard, 99.5%