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A whole-cell bioreporter approach for the genotoxicity assessment of bioavailability of toxic compounds in contaminated soil in China.

Environmental pollution (Barking, Essex : 1987) (2014-09-23)
Yizhi Song, Bo Jiang, Sicong Tian, Hui Tang, Zengjun Liu, Chuan Li, Jianli Jia, Wei E Huang, Xu Zhang, Guanghe Li
ABSTRACT

A whole-cell bacterial bioreporter Acinetobacter baylyi strain ADP1_recA_lux that responds to genotoxins was employed to directly assess the adverse effects of the bioavailable fraction of mitomycin C (MMC), benzo[a]pyrene (BaP), chromium (VI) and lead (II) in amended soils and soil samples from two fragile areas in China without soil pre-treatment. The amended soils containing pollutants with the concentrations as low as 0.4 mg/kg MMC, 0.5 mg/kg BaP, 520 mg/kg Cr (VI) and 2072 mg/kg Pb (II) were found to be toxic. Soil particle-associated pollutants accounted for 86%, 100%, 29%, and 92% of the genotoxicity in the MMC, BaP, Cr (VI), and Pb (II) amended soil, respectively. The soils from contaminated sites were also valid to be genotoxic. The results suggest both free and soil particle-associated pollutants are bioavailable to soil organisms and a solid-phase contact bioreporter assay to soil contamination could provide a rapid screening tool for environmental risk assessment.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Benzo[a]pyrene, ≥96% (HPLC)
Sigma-Aldrich
Chromium, chips, 99.995% trace metals basis
Supelco
Benzo[a]pyrene, analytical standard, for environmental analysis
Sigma-Aldrich
Chromium, powder, ≥99% trace metals basis, <45 μm
Supelco
Benzo[a]pyrene, certified reference material, TraceCERT®
Supelco
Benzo[a]pyrene solution, certified reference material, TraceCERT®, 1000 μg/mL in acetone
Supelco
Benzo[a]pyrene solution, certified reference material, TraceCERT®, 200 μg/mL in methylene chloride
Sigma-Aldrich
Chromium, powder, 99.5%, −100 mesh
Supelco
Benzo[a]pyrene solution, 100 μg/mL in cyclohexane, analytical standard
Supelco
Benzo[a]pyrene solution, certified reference material, 100 μg/mL in methylene chloride
Sigma-Aldrich
Chromium, chips, thickness ~2 mm, 99.5%
Chromium, rod, 10mm, diameter 5.0mm, 99.7+%
Chromium, foil, light tested, 25x25mm, thickness 0.05mm, permanent polyester support, 99.99+%
Chromium, foil, light tested, 25x25mm, thickness 0.075mm, permanent polyester support, 99.99+%
Chromium, foil, not light tested, 100x100mm, thickness 0.0125mm, permanent polyester support, 99.99+%
Chromium, foil, not light tested, 100x100mm, thickness 0.015mm, permanent polyester support, 99.99+%
Chromium, foil, not light tested, 100x100mm, thickness 0.01mm, permanent polyester support, 99.99+%
Chromium, foil, not light tested, 100x100mm, thickness 0.025mm, permanent polyester support, 99.99+%
Chromium, foil, not light tested, 100x100mm, thickness 0.02mm, permanent polyester support, 99.99+%
Chromium, foil, not light tested, 100x100mm, thickness 0.03mm, permanent polyester support, 99.99+%
Chromium, foil, not light tested, 100x100mm, thickness 0.04mm, permanent polyester support, 99.99+%
Chromium, foil, not light tested, 25x25mm, thickness 0.0015mm, permanent polyester support, 99.99+%
Chromium, foil, not light tested, 25x25mm, thickness 0.001mm, permanent polyester support, 99.99+%
Chromium, foil, not light tested, 25x25mm, thickness 0.0025mm, permanent polyester support, 99.99+%
Chromium, foil, not light tested, 25x25mm, thickness 0.002mm, permanent polyester support, 99.99+%
Chromium, foil, not light tested, 25x25mm, thickness 0.003mm, permanent polyester support, 99.99+%
Chromium, foil, not light tested, 25x25mm, thickness 0.004mm, permanent polyester support, 99.99+%
Chromium, foil, not light tested, 25x25mm, thickness 0.005mm, permanent polyester support, 99.99+%
Chromium, foil, not light tested, 25x25mm, thickness 0.007mm, permanent polyester support, 99.99+%
Chromium, foil, not light tested, 25x25mm, thickness 0.009mm, permanent polyester support, 99.99+%