• Home
  • Search Results
  • Isolation and characterization of oxalotrophic bacteria from tropical soils.

Isolation and characterization of oxalotrophic bacteria from tropical soils.

Archives of microbiology (2014-11-10)
Daniel Bravo, Olivier Braissant, Guillaume Cailleau, Eric Verrecchia, Pilar Junier
ABSTRACT

The oxalate-carbonate pathway (OCP) is a biogeochemical set of reactions that involves the conversion of atmospheric CO2 fixed by plants into biomass and, after the biological recycling of calcium oxalate by fungi and bacteria, into calcium carbonate in terrestrial environments. Oxalotrophic bacteria are a key element of this process because of their ability to oxidize calcium oxalate. However, the diversity and alternative carbon sources of oxalotrophs participating to this pathway are unknown. Therefore, the aim of this study was to characterize oxalotrophic bacteria in tropical OCP systems from Bolivia, India, and Cameroon. Ninety-five oxalotrophic strains were isolated and identified by sequencing of the 16S rRNA gene. Four genera corresponded to newly reported oxalotrophs (Afipia, Polaromonas, Humihabitans, and Psychrobacillus). Ten strains were selected to perform a more detailed characterization. Kinetic curves and microcalorimetry analyses showed that Variovorax soli C18 has the highest oxalate consumption rate with 0.240 µM h(-1). Moreover, Streptomyces achromogenes A9 displays the highest metabolic plasticity. This study highlights the phylogenetic and physiological diversity of oxalotrophic bacteria in tropical soils under the influence of the oxalate-carbonate pathway.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Magnesium sulfate, anhydrous, ReagentPlus®, ≥99.5%
Sigma-Aldrich
Calcium carbonate, ACS reagent, ≥99.0%, powder
Sigma-Aldrich
Oxalic acid, 98%
Sigma-Aldrich
Oxalic acid, puriss. p.a., anhydrous, ≥99.0% (RT)
Sigma-Aldrich
Activated charcoal, DARCO®, −100 mesh particle size, powder
Sigma-Aldrich
Calcium carbonate, ACS reagent, chelometric standard, 99.95-100.05% dry basis
Supelco
Activated charcoal, powder
Sigma-Aldrich
Magnesium sulfate, BioReagent, suitable for cell culture, suitable for insect cell culture
Sigma-Aldrich
Calcium carbonate, ReagentPlus®
Sigma-Aldrich
Magnesium sulfate, anhydrous, reagent grade, ≥97%
Sigma-Aldrich
Activated charcoal, powder, -100 particle size (mesh), decolorizing
Sigma-Aldrich
Oxalic acid, ReagentPlus®, ≥99%
Sigma-Aldrich
Magnesium sulfate, anhydrous, free-flowing, Redi-Dri, ReagentPlus®, ≥99.5%
Sigma-Aldrich
Calcium carbonate, powder, ≤50 μm particle size, 98%
Sigma-Aldrich
Activated charcoal, suitable for cell culture, suitable for plant cell culture
Sigma-Aldrich
Sodium oxalate, puriss. p.a., ACS reagent, ≥99.5% (RT)
Sigma-Aldrich
Sodium oxalate, ACS reagent, ≥99.5%
Sigma-Aldrich
Magnesium sulfate solution, for molecular biology, 1.00 M±0.04 M
Sigma-Aldrich
Magnesium sulfate, puriss. p.a., drying agent, anhydrous, ≥98.0% (KT), powder (very fine)
Sigma-Aldrich
Carbon, glassy, spherical powder, 2-12 μm, 99.95% trace metals basis
Supelco
Activated charcoal, puriss. p.a., powder
Sigma-Aldrich
Carbon, nanopowder, <100 nm particle size (TEM)
Sigma-Aldrich
Activated charcoal, DARCO®, 20-40 mesh particle size, granular
Supelco
Sodium oxalate, certified reference material for titrimetry, certified by BAM, ≥99.5%
Sigma-Aldrich
Magnesium sulfate, anhydrous, free-flowing, Redi-Dri, reagent grade, ≥97%
Sigma-Aldrich
Activated Charcoal Norit®, Norit® SX ultra, from peat, corresponds U.S. Food chemicals codex (3rd Ed.), steam activated and acid washed, highly purified, powder
Sigma-Aldrich
Calcium carbonate, BioXtra, ≥99.0%
Sigma-Aldrich
Oxalic acid, purified grade, 99.999% trace metals basis
Sigma-Aldrich
Magnesium sulfate solution, BioUltra, for molecular biology
Supelco
Calcium carbonate, Pharmaceutical Secondary Standard; Certified Reference Material