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A GC-FID validated method for the quality control of Eucalyptus globulus raw material and its pharmaceutical products, and gc-ms fingerprinting of 12 Eucalyptus species.

Natural product communications (2015-01-30)
Paula Carolina Pires Buenoa, Milton Groppo Junior, Jairo Kenupp Bastos
ABSTRACT

In this work we have validated a method to standardize and control the quality of Eucalyptus globulus raw material and phytomedicines containing either the essential oil or the fluid extract of this plant in the final formulation. Internal standardization provided a simple, fast, and reproducible GC-FID analytical method that accurately quantified 1,8-cineol in different E. globulus sub-products, such as its essential oil, dried leaves, fluid extract, and syrup. In addition, GC-MS identification of the main compounds ofE. globulus species afforded fingerprints for the qualitative analysis of different Eucalyptus species.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Eucalyptol, 99%
Supelco
Eucalyptol, analytical standard
Sigma-Aldrich
Eucalyptol, natural, ≥99%, FCC, FG
Supelco
1,8-Cineole, primary reference standard
Cineole, European Pharmacopoeia (EP) Reference Standard
Supelco
Eucalyptol, certified reference material, TraceCERT®