• Home
  • Search Results
  • Is plasma cortisol response to stress in rainbow trout regulated by catecholamine-induced hyperglycemia?

Is plasma cortisol response to stress in rainbow trout regulated by catecholamine-induced hyperglycemia?

General and comparative endocrinology (2014-04-17)
Manuel Gesto, Cristina Otero-Rodiño, Marcos A López-Patiño, Jesús M Míguez, José L Soengas, Marta Conde-Sieira
ABSTRACT

Based on previous studies we hypothesize that under stress conditions catecholamine-induced hyperglycemia contributes to enhance cortisol production in head kidney of rainbow trout. Therefore, treatment with propranolol (β-adrenoceptor blocker) should reduce the hyperglycemia elicited by stress and, therefore, we expected reduced glucosensing response and cortisol production in head kidney. Propranolol treatment was effective in blocking most of the effects of catecholamines in liver energy metabolism resulting in a lower glycemia in stressed fish. The decreased glycemia of stressed fish treated with propranolol was observed along with reduced transcription of genes involved in the cortisol synthetic pathway, which supports our hypothesis. However, changes in putative glucosensing parameters assessed in head kidney were scarce and in general did not follow changes noted in glucose levels in plasma. Furthermore, circulating cortisol levels did not change in parallel with changes in glycemia. As a whole, the present results suggest that glycemia could participate in the regulation of cortisol synthetic pathways but other factors are also likely involved. Propranolol effects on trout stress response were different depending on time passed after stress onset; the direct or indirect involvement of catecholaminergic response in the regulation of cortisol production and release deserves further investigation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Hydrocortisone, BioReagent, suitable for cell culture
Sigma-Aldrich
Protease Inhibitor Cocktail powder, for general use, lyophilized powder
Sigma-Aldrich
2-Phenoxyethanol, ≥99%
Sigma-Aldrich
Hydrocortisone, ≥98% (HPLC)
Supelco
2-Phenoxyethanol, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Hydrocortisone, γ-irradiated, powder, BioXtra, suitable for cell culture
Supelco
Hydrocortisone, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
2-Phenoxyethanol, analytical standard
Sigma-Aldrich
Ethylene glycol monophenyl ether, ≥90%
Hydrocortisone, European Pharmacopoeia (EP) Reference Standard
USP
Hydrocortisone, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Hydrocortisone, meets USP testing specifications
Hydrocortisone for peak identification, European Pharmacopoeia (EP) Reference Standard
Supelco
Hydrocortisone, VETRANAL®, analytical standard
Hydrocortisone, British Pharmacopoeia (BP) Assay Standard