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2-Deoxy-d-glucose attenuates sevoflurane-induced neuroinflammation through nuclear factor-kappa B pathway in vitro.

Toxicology in vitro : an international journal published in association with BIBRA (2014-06-08)
Qingxiu Wang, Yupeng Zhao, Min Sun, Sheng Liu, Baolin Li, Lei Zhang, Longqiu Yang
ABSTRACT

Sevoflurane, one of the most commonly used anesthetics in clinic, induced neuroinflammation and caused cognitive impairment. 2-deoxy-d-glucose (2-DG) is a synthetic analogue of glucose and is clinically used in medical imaging safely. We examined the effect of 2-DG on sevoflurane-induced neuroinflammation in the mouse primary microglia cells. Mouse microglia cells were treated with 4.1% sevoflurane for 6h to examine the expression of interleukin (IL)-6 and tumor necrosis factor (TNF-α) and activation of nuclear factor-kappa B (NF-κB). Pyrrolidine dithiocarbamate (PDTC) or 2-DG was used 1h before sevoflurane treatment. In the present study, we found that sevoflurane increased level of IL-6 and TNF-α through activating NF-κB signaling, and that 2-DG reduced sevoflurane-induced increase in IL-6 and TNF-α and nuclear NF-κB in microglia cells. Our data suggests that NF-κB signaling pathway could be a target for sevoflurane-induced neuroinflammation and 2-DG might be a potential therapy to prevent or treat sevoflurane-induced neuroinflammation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2-Deoxy-D-glucose, ≥98% (GC), crystalline
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Human IL-6 ELISA Kit, for serum, plasma, cell culture supernatant and urine
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Rat Interleukin-6 ELISA Kit, for for cell lysate and tissue lysate
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Human IL-6 ELISA Kit, for cell and tissue lysates
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Rat IL-6 ELISA Kit, for serum, plasma and cell culture supernatants
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Mouse IL-6 R ELISA Kit, for serum, plasma and cell culture supernatant
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Ammonium pyrrolidinedithiocarbamate, ~99%
Sevoflurane, European Pharmacopoeia (EP) Reference Standard
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Human IL-6 sR ELISA Kit, for serum, plasma, cell culture supernatant and urine
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Mouse IL-6 ELISA Kit, for cell and tissue lysates
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Mouse IL-6 ELISA Kit, for serum, plasma and cell culture supernatant