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  • Human embryo features that influence the success of cryopreservation with the use of 1,2 propanediol.

Human embryo features that influence the success of cryopreservation with the use of 1,2 propanediol.

Fertility and sterility (1985-11-01)
B Lassalle, J Testart, J P Renard
ABSTRACT

Eighty-five human embryos fertilized in vitro were frozen and thawed with 1,2 propanediol as a cryoprotective agent. The effects of viability of in vitro culture duration, stage, and morphologic appearance of embryos were examined after thawing. Survival was higher for 2-day-old embryos than for 3-day-old embryos (56% versus 18%) and for 2-, 4-, and 8-cell embryos than for intermediate-cleavage-stage embryos (67% versus 22%). Among 19 regular-cell-size embryos at the 2-, 4-, and 8-cell stage, 15 (79%) kept 50% or more of their initial number of blastomeres after thawing and 9 were intact. The average viability of all 2-day-old frozen-thawed embryos can be estimated at 19%.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
1,2-Propanediol, meets analytical specification of Ph. Eur., BP, USP, ≥99.5%
Sigma-Aldrich
1,2-Propanediol, puriss. p.a., ACS reagent, ≥99.5% (GC)