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Simple, rapid, and quantitative release of periplasmic proteins by chloroform.

Journal of bacteriology (1984-12-01)
G F Ames, C Prody, S Kustu
ABSTRACT

We introduce a method by which periplasmic proteins can be released rapidly, simply, and quantitatively by treating cells with chloroform. All the amino acid-binding proteins tested maintained their activity during chloroform treatment. This method makes practical the analysis of the periplasmic protein complement of a large number of strains.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Chloroform, puriss. p.a., reag. ISO, reag. Ph. Eur., 99.0-99.4% (GC)
Sigma-Aldrich
Chloroform, contains ethanol as stabilizer, meets analytical specification of BP, 99-99.4% (GC)