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Photoswitchable fluorescent proteins for superresolution fluorescence microscopy circumventing the diffraction limit of light.

Methods in molecular biology (Clifton, N.J.) (2013-10-11)
Susana Rocha, Herlinde De Keersmaecker, Hiroshi Uji-i, Johan Hofkens, Hideaki Mizuno
ABSTRACT

In the last two decades, fluorescent proteins became an indispensable tool to noninvasively label a protein in living cells. The discovery of photoswitchable fluorescent proteins expanded the applications of the fluorescent proteins to techniques such as molecular tracking and highlighting on a microscope. Recently, a new microscopic modality to achieve a superresolution circumventing the diffraction limit of light (photoactivated localization microscopy, PALM) has been developed based on the photoswitchable fluorescent proteins. Here we introduce a basic protocol of PALM through the visualization of actin bundles with superresolution.

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Roche
X-tremeGENE 9 DNA Transfection Reagent, Polymer reagent for transfecting common cell lines

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