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A chemically labeled cytotoxic agent: two-photon fluorophore for optical tracking of cellular pathway in chemotherapy.

Proceedings of the National Academy of Sciences of the United States of America (1999-09-29)
X Wang, L J Krebs, M Al-Nuri, H E Pudavar, S Ghosal, C Liebow, A A Nagy, A V Schally, P N Prasad
ABSTRACT

Chemotherapy is commonly used in the treatment of cancers. However, the mechanism of action of many of these agents is not well understood. We present the synthesis of a two-photon fluorophore (C625) and its biological application when chemically linked to a chemotherapeutic agent (AN-152). By using two-photon laser-scanning microscopy, the drug:fluorophore conjugate can be observed directly as it interacts with receptor-positive cell lines. The results of this project visually show the receptor-mediated entry of AN-152 into the cell cytoplasm and subsequently into the nucleus. These observations will allow for better understanding of the drug's therapeutic mechanism, which is a subject of ongoing research aimed at improving present methods for cancer therapy.

MATERIALS
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Brand
Product Description

Sigma-Aldrich
N,N-Dimethylformamide, anhydrous, 99.8%