• Response to Nodal morphogen gradient is determined by the kinetics of target gene induction.

Response to Nodal morphogen gradient is determined by the kinetics of target gene induction.

eLife (2015-04-15)
Julien Dubrulle, Benjamin M Jordan, Laila Akhmetova, Jeffrey A Farrell, Seok-Hyung Kim, Lilianna Solnica-Krezel, Alexander F Schier

Morphogen gradients expose cells to different signal concentrations and induce target genes with different ranges of expression. To determine how the Nodal morphogen gradient induces distinct gene expression patterns during zebrafish embryogenesis, we measured the activation dynamics of the signal transducer Smad2 and the expression kinetics of long- and short-range target genes. We found that threshold models based on ligand concentration are insufficient to predict the response of target genes. Instead, morphogen interpretation is shaped by the kinetics of target gene induction: the higher the rate of transcription and the earlier the onset of induction, the greater the spatial range of expression. Thus, the timing and magnitude of target gene expression can be used to modulate the range of expression and diversify the response to morphogen gradients.

Product Number
Product Description

Cycloheximide, Biotechnology Performance Certified
Cycloheximide, PESTANAL®, analytical standard
Cycloheximide solution, Ready-Made Solution, microbial, 100 mg/mL in DMSO, Suitable for cell culture
Cycloheximide, from microbial, ≥94% (TLC)
Cycloheximide, ≥90% (HPLC)
Monoclonal ANTI-FLAG® M2 antibody produced in mouse, clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)
Cycloheximide solution, 0.1%, suitable for microbiology
Anti-SMAD2 antibody produced in rabbit, IgG fraction of antiserum
SB-505124 hydrochloride hydrate, ≥98% (HPLC)