MilliporeSigma
  • Home
  • Search Results
  • Correlations between PAH bioavailability, degrading bacteria, and soil characteristics during PAH biodegradation in five diffusely contaminated dissimilar soils.

Correlations between PAH bioavailability, degrading bacteria, and soil characteristics during PAH biodegradation in five diffusely contaminated dissimilar soils.

Environmental science and pollution research international (2014-03-29)
M Crampon, F Bureau, M Akpa-Vinceslas, J Bodilis, N Machour, F Le Derf, F Portet-Koltalo
ABSTRACT

The natural biodegradation of seven polycyclic aromatic hydrocarbons (PAHs) by native microorganisms was studied in five soils from Normandy (France) from diffusely polluted areas, which can also pose a problem in terms of surfaces and amounts of contaminated soils. Bioavailability tests using cyclodextrin-based extractions were performed. The natural degradation of low molecular weight (LMW) PAHs was not strongly correlated to their bioavailability due to their sorption to geosorbents. Conversely, the very low degradation of high molecular weight (HMW) PAHs was partly correlated to their poor availability, due to their sorption on complexes of organic matter and kaolinites or smectites. A principal component analysis allowed us to distinguish between the respective degradation behaviors of LMW and HMW PAHs. LMW PAHs were degraded in less than 2-3 months and were strongly influenced by the relative percentage of phenanthrene-degrading bacteria over total bacteria in soils. HMW PAHs were not significantly degraded, not only because they were less bioavailable but also because of a lack of degrading microorganisms. Benzo[a]pyrene stood apart since it was partly degraded in acidic soils, probably because of a catabolic cooperation between bacteria and fungi.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Benzo[a]pyrene, ≥96% (HPLC)
Supelco
Fluoranthene, analytical standard
Fluoranthene, BCR®, certified reference material
Sigma-Aldrich
Phenanthrene, 98%
Supelco
Benzo[a]pyrene, certified reference material, TraceCERT®
Sigma-Aldrich
Fluoranthene, 98%
Supelco
Phenanthrene, analytical standard, for environmental analysis
Supelco
Fluoranthene, analytical standard
Supelco
Benzo[a]pyrene, analytical standard, for environmental analysis
Sigma-Aldrich
Phenanthrene, sublimed grade, ≥99.5%
Supelco
Phenanthrene, certified reference material, TraceCERT®
Supelco
Fluoranthene, certified reference material, TraceCERT®
Supelco
Benzo[a]pyrene solution, 100 μg/mL in cyclohexane, analytical standard
Supelco
Benzo[b]fluoranthene, analytical standard
Supelco
Pyrene, analytical standard
Supelco
Benzo[a]pyrene solution, certified reference material, 100 μg/mL in methylene chloride
Supelco
Pyrene, certified reference material, TraceCERT®
Sigma-Aldrich
Benzo[k]fluoranthene, for fluorescence, ≥99%
Sigma-Aldrich
Pyrene, 98%
Sigma-Aldrich
Benzo[b]fluoranthene, 98%
Benzo[k]fluoranthene, BCR®, certified reference material
Pyrene, BCR®, certified reference material
Sigma-Aldrich
Pyrene, puriss. p.a., for fluorescence, ≥99.0% (GC)
Sigma-Aldrich
Pyrene, sublimed grade, 99%
Supelco
Benzo[k]fluoranthene, certified reference material, TraceCERT®
Supelco
Benzo[b]fluoranthene, certified reference material, TraceCERT®
Supelco
Benzo[a]pyrene solution, certified reference material, TraceCERT®, 1000 μg/mL in acetone
Supelco
Benzo[a]pyrene solution, certified reference material, TraceCERT®, 200 μg/mL in methylene chloride