• Home
  • Search Results
  • Role of VPAC2 receptor in monocrotaline-induced pulmonary hypertension in rats.

Role of VPAC2 receptor in monocrotaline-induced pulmonary hypertension in rats.

Journal of applied physiology (Bethesda, Md. : 1985) (2014-06-21)
Motokazu Koga, Yusuke Mizuno, Itaru Watanabe, Hiromasa Kawakami, Takahisa Goto
ABSTRACT

Pulmonary hypertension (PH) is associated with significant morbidity and mortality. Vasoactive intestinal peptide (VIP) and pituitary adenylyl cyclase activating peptide (PACAP) have pulmonary vasodilatory and positive inotropic effects via receptors VPAC1 and VPAC2, which possess a similar affinity for both peptides, and PAC1, a PACAP-preferring receptor. VIP is a promising option for PH treatment; however, various physiological effects of VIP have limited its clinical use. We investigated the effects of VPAC1 and VPAC2 selective agonists VIP and PACAP to explore more appropriate means of treatment for PH. We examined hemodynamic changes in right ventricular systolic pressure (RVSP), systemic blood pressure (SBP), total pulmonary resistance index (TPRI), total systemic resistance index, and cardiac index (CI) in response to their agonists with monocrotaline (MCT)-induced PH and explored involvement of VIP/PACAP expression and receptors in PH. Sprague-Dawley rats were divided into the MCT group (administered MCT 60 mg/kg) and control group. In MCT-induced PH, decreased VIP and PACAP were associated with upregulation of VPAC1, VPAC2, and PAC1 in lung tissues. Intravenous injection of VPAC2-selective agonist BAY 55-9837 and VIP, but not [Ala(11,22,28)]VIP, improved the CI. The decrease in SBP with VPAC2 agonist was significantly less than that in the control. Although they decreased SBP, these agonists hardly affected RVSP in the control. Activation of VPAC2 receptor with BAY 55-9837 effectively improved RVSP, TPRI, and CI in MCT-induced PH, suggesting a VPAC2 agonist as a possible promising treatment for PH.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
L-Lysine monohydrochloride, reagent grade, ≥98% (HPLC)
Sigma-Aldrich
L-Lysine monohydrochloride, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Supelco
L-Lysine monohydrochloride, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
L-Lysine monohydrochloride, BioUltra, ≥99.5% (AT)
Lysine hydrochloride, European Pharmacopoeia (EP) Reference Standard
Supelco
L-Lysine hydrochloride solution, 100 mM amino acid in 0.1 M HCl, analytical standard
Supelco
L-Lysine monohydrochloride, certified reference material, TraceCERT®
Sigma-Aldrich
Anti-VIP antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-PSMG1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-ADCYAP1R1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-ADCYAP1R1 antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-VIPR1 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Monoclonal Anti-KLF6, (N-terminal) antibody produced in mouse, clone 3C4, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Anti-CPAMD8 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-KLF6 antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Monoclonal Anti-DSCR2 antibody produced in mouse, clone 4F2, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Monoclonal Anti-ADCYAP1R1 antibody produced in mouse, clone 2B12, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Monoclonal Anti-KLF6 antibody produced in mouse, clone 1A9, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Anti-ADCYAP1R1 antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-ADCYAP1R1 antibody produced in rabbit, affinity isolated antibody