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Purification and characterization of cocoonase from the silkworm Bombyx mori.

Bioscience, biotechnology, and biochemistry (2014-07-19)
Hisayoshi Fukumori, Satoshi Teshiba, Yuichi Shigeoka, Kohji Yamamoto, Yutaka Banno, Yoichi Aso
ABSTRACT

Cocoonase (CCN) which facilitates the degradation of a cocoon is recognized as a trypsin-like serine protease. In this study, CCN from the silkworm Bombyx mori was purified and comprehensively characterized. Its activity was maximal at about pH 9.8. It was stable above pH 3.4 at 4 °C and below 50 °C at pH 7.5. CuSO₄, FeSO₄, and ZnSO₄ showed inhibitory effects on CCN, but other salts improved activity. Typical trypsin inhibitors inhibited CCN, but the relative inhibitory activities were much lower than those against bovine trypsin. An extract of cocoon shells inhibited trypsin, but it was only slightly inhibitory against CCN. There were significant differences in catalytic efficiencies and substrate specificities as between CCN and bovine trypsin.

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