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Antimicrobial and enzyme inhibitory activities of the constituents of Plectranthus madagascariensis (Pers.) Benth.

Journal of enzyme inhibition and medicinal chemistry (2014-02-11)
Renata Kubínová, Radka Pořízková, Alice Navrátilová, Oldřich Farsa, Zuzana Hanáková, Adriana Bačinská, Alois Cížek, Marie Valentová
ABSTRACT

Plectranthus madagascariensis is used as a traditional medicine in Southern Africa. In search of compounds and activities supporting the medicinal use, the chemical profile of the methanolic extract was studied by high-performance liquid chromatography with diode array detection (HPLC-DAD). Four major constituents were isolated and identified as rosmarinic acid (1), 7β,6β-dihydroxyroyleanone (2), 7β-acetoxy-6β-hydroxyroyleanone (3) and coleon U quinone (4). The two abietane diterpenoids (2 and 3) were isolated for the first time from this species. Antimicrobial, cholinesterase and α-glucosidase inhibitory activities of these compounds were studied. The compounds exhibited inhibitory activity on α-glucosidase with IC50 values from 33 to 275 μM. Abietanes showed potent antibacterial activity against Staphylococcus aureus and Enterococcus faecalis.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
5,5′-Dithiobis(2-nitrobenzoic acid), ≥98%, BioReagent, suitable for determination of sulfhydryl groups
Sigma-Aldrich
5,5′-Dithiobis(2-nitrobenzoic acid), ReagentPlus®, 99%
Sigma-Aldrich
Rosmarinic acid, ≥98% (HPLC), from Rosemarinus officinalis L.
Sigma-Aldrich
Acetylthiocholine iodide, ≥98% (TLC), powder or crystals
Sigma-Aldrich
Acarbose, ≥95%
Sigma-Aldrich
Rosmarinic acid, 96%
Supelco
Acarbose, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Acetylthiocholine iodide, ≥99.0% (AT)
Sigma-Aldrich
Butyrylthiocholine iodide, ≥98%
Sigma-Aldrich
Butyrylthiocholine iodide, puriss., ≥99.0% (AT)
Rosmarinic acid, European Pharmacopoeia (EP) Reference Standard
Rosmarinic acid, primary reference standard
Acarbose, European Pharmacopoeia (EP) Reference Standard