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Using chemical desorption of PAHs from sediment to model biodegradation during bioavailability assessment.

Journal of hazardous materials (2014-09-28)
Jelena M Spasojević, Snežana P Maletić, Srđan D Rončević, Dragan V Radnović, Dragana I Cučak, Jelena S Tričković, Božo D Dalmacija
ABSTRACT

This work compares the biodegradation potential of four polycyclic aromatic hydrocarbons (PAH) (phenanthrene, pyrene, chrysene and benzo(a)pyrene, chosen as representatives of the 3, 4 and 5 ring PAHs) with their desorption from sediment by XAD4 resin and methyl-β-cyclodextrin (MCD). The biodegradation study was conducted under various conditions (biostimulation, bioaugmentation and their combination). The results show that total PAH removal in all treatments except biostimulation gave similar results, whereby the total amount of PAHs was decreased by about 30-35%. The desorption experiment showed that XAD4 desorbed a greater fraction of phenanthrene (77% versus 52%), and benzo(a)pyrene (44% versus 25%) than MCD. The results for four ring PAHs were similar for both desorption agents (about 30%). Comparing the maximum biodegraded amount of each PAH with the rapidly desorbed XAD4 and MCD fraction, XAD4 was found to correlate better with biodegradation for the high molecular PAHs (pyrene, chrysene, benzo(a)pyrene), although it overestimated the availability of phenanthrene. In contrast, MCD showed better correlation with the biodegradation of low molecular weight PAHs.

MATERIALS
Product Number
Brand
Product Description

Supelco
Pyrene, analytical standard
Supelco
Pyrene, certified reference material, TraceCERT®
Sigma-Aldrich
Pyrene, 98%
Pyrene, BCR®, certified reference material
Sigma-Aldrich
Pyrene, puriss. p.a., for fluorescence, ≥99.0% (GC)
Sigma-Aldrich
Pyrene, sublimed grade, 99%
Sigma-Aldrich
Phenanthrene, 98%
Chrysene, BCR®, certified reference material
Supelco
Chrysene, analytical standard
Sigma-Aldrich
Phenanthrene, sublimed grade, ≥99.5%
Supelco
Chrysene, certified reference material, TraceCERT®
Supelco
Phenanthrene, analytical standard, for environmental analysis
Supelco
Phenanthrene, certified reference material, TraceCERT®